The resistance of 10 entries of melons (Cucumis melo) to powdery mildew, caused by Sphaerotheca fuliginea race 1, was evaluated by means of a leaf disk assay. Disks (9 mm in diameter), removed from fully expanded leaves, were placed in petri dishes containing water agar amended with 25 μg/ml of benzimidazole and maintained at 25 C. Disks were inoculated by applying a 10-μl drop of a water suspension of conidia or by blowing air over an infected plant toward the test disk. Differences in inoculation efficacy between the two methods were insignificant. Infection efficacy and sporulation were greater for the adaxial position of the disk (.)
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R. Cohen (1993) studied this question.
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