With 2 figures Abstract In most economically important dioecious plants such as Simmondsia chinensis (Jojoba), sex identification based on flowering can only be made after 1–5 years of plantation. Identification of sex‐associated molecular markers for ascertaining sex at seedling stage is a desirable prerequisite for optimal plantation and economical benefits. Jojoba is being developed as a plantation crop in the arid regions of Western India due to the commercial benefits of the unique waxy content of its seeds. In the present study, sex‐linked DNA markers have been identified in S. chinensis using the AFLP technique. From sixteen primers combinations used for AFLP analysis, two combinations EcoRI‐TAC/MseI‐GCG and EcoRI‐GC/MseI‐GCG resulted in amplification of sex‐linked markers. Two male‐specific markers of ∼525 bp and ∼325 bp were identified using the primer combinations EcoRI‐GC/MseI‐GCG and EcoRI‐TAC/MseI‐GCG, respectively. A female‐specific marker of ∼270 bp was also identified with the primer combination EcoRI‐TAC/MseI‐GCG. In future, SCAR markers developed from these sex‐specific markers will provide an effective and reliable method for sex identification.
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Agarwal et al. (2010) studied this question.
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