Chitosan-g-polyaniline: a creatine amidinohydrolase immobilization matrix for creatine biosensor

A novel matrix composed of chitosan-graft-polyaniline (CHIT-g-PANI) was electrochemically prepared to investigate the immobilization of creatine amidinohydrolase (CAH).CAH enzyme was covalently immobilized with the CHIT-g-PANI matrix using glutaraldehyde as a linker.The resulting CAH/CHIT-g-PANI biomatrix was characterized with Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), contact angle measurement and cyclic voltammetry (CV) taking CHIT-g-PANI as a reference.The influence of various parameters on CAH enzyme activity within the matrix was investigated including pH, temperature, and time.The Michaelis-Menten constant and apparent activities for the CAH enzyme were calculated to be 0.51 mM and 83.59 mg/cm 2 , respectively; indicating CHIT-g-PANI matrix has a high affinity to immobilize CAH enzyme.