Anti-Peptide Antibodies Directed Against Omega-Gliadins for the Detection of Sequences from Bread and Durum Wheats

This paper presents the characterisation of polyclonal antibodies directed against two different N-terminal sequences of ω-gliadins. Both antisera recognised specifically the corresponding omega-gliadin fractions but showed different reactivities against ω-gliadins extracted from bread and durum wheats. Antibodies directed against the 'SRLL' type (ω5 type) recognised several ω-gliadin components in bread and durum wheat extracts whereas antibodies directed against the 'AREL' type (ω2 type) reacted specifically with an ω-gliadin component from bread wheat. The narrow specificity of this antiserum made it potentially interesting for the detection of bread wheat additions in durum wheat pasta. Moreover, the reactivity of this antiserum was not modified by an increase in drying temperature of pasta.