A novel nuclear magnetic resonance (NMR) assay has been developed to monitor lipase‐catalyzed esterification reactions without the need to extract and purify the individual components. The technique measures ratios of ester:alcohol signals and has been shown to be both efficient and reproducible. The assay has proven useful as a quick screen for the effect of varying conditions on the extent of esterification in various biphasic solvent systems and can be applied to both saturated and unsaturated long‐chain fatty acid and alcohol substrates. The NMR ratio technique has been used to quantitate the extent of reaction in theCandida cylindracea lipase‐catalyzed synthesis of oleyl palmitate, stearyl palmitate, oleyl oleate, oleylgamma‐linolenate and oleyl linoleate. The identity of these products was confirmed by high‐resolution mass spectrometry.
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OʼConnor et al. (1992) studied this question.
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