Growth characteristicsand cell composition of Alcaligenes eutrophus under limitation of gaseous substrates were evaluated in a chemostatically controlled continuous culture.Yield coefficients, especially YH2, and cell composition varied conspicuously with the change of growth limiting gas from hydrogen to oxygen.In 02-limitation, YH2 was 1.5 times higher than that in H2-limitation but the content of protein in cell decreased.With feeding the gas-mixture containing H2: 02: CO2=3: 1: 0.4, simultaneous limitation of three gases was suspected to occur.With this feeding ratio of gases, highest productivity of cell mass and efficient utilization of gaseous substrates were obtained.
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Morinaga et al. (1978) studied this question.