Answers are grounded in this paper and cite their sources.
A marker assisted selection protocol is presented that allows for the generation of congenic or consomic strains derived from a C57BL/6J:129S6/SvEvTac mixed strain background. The protocol uses defined primer pairs to generate amplicons that can be distinguished by non-denaturing agarose electrophoresis. Use of this application should result in substantial savings in time, effort, and cost for investigators in all areas of transgenic mouse research.
No takes yet. Share an insight, caveat, or question.
Estill et al. (2000) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: