Plasma samples from nine White Leghorn hens collected over six different intervals during the laying cycle contained high concentrations of estradiol-17β-3-sulfate with an overall mean of 1.24 pmol/ml (464 pg/ml) versus .36 pmol/ml (98 pg/ml) for estradiol-17β. In contrast, the mean concentration of estrone sulfate was only .59 pmol/ml (219 pg/ml) as compared to .41 pmol/ml (111 pg/ml) for estrone.
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Tsang et al. (1981) studied this question.
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