Key result
Extended-U3-deleted nonintegrating lentiviral vectors improve sustained episomal transgene expression in mouse liver.
Population
In vitro and in vivo models including mouse liver and rat brain corpus callosum
Comparison
Nonintegrating SIN lentiviral vectors with longer U3 deletion vs integrating vectors and nonintegrating vectors without longer U3 deletion
Design
Preclinical study
Authors
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Supports nonintegrating lentiviral vectors for retinal models; leaves open human translation and safety.
A longer U3 deletion in nonintegrating lentiviral vectors improves transgene expression in vivo, particularly in the mouse liver, overcoming previous limitations of low transduction levels.
Bayer et al. (2008) studied this question. SIN nonintegrating lentiviral vectors with a longer U3 deletion vs. Integrating lentiviral vectors and vectors without longer U3 deletion was evaluated on Transgene expression levels. Nonintegrating lentiviral vectors with a longer U3 deletion showed improved transgene expression, with potent and sustained episomal expression demonstrated in the mouse liver.
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