Sycamore and soybean cell suspensions were subjected to osmotic stress by culturing for one passage in media supplemented with 6 per cent mannitol or sorbitol. The effects on growth were to reduce cell number and biomass (d. wt) production throughout the culture period by about 30 per cent. Ultrastructural studies at the early exponential phase of culture growth indicated similar reductions in cell wall thickness in both species. Osmotically stressed sycamore cells became less vacuolate, but no such change occurred in stressed soybean cells.
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Pritchard et al. (1986) studied this question.