Studies on the Inhibition of Brain Phospho‐fructokinase by Oleate and the Determination of Fatty Acids in Normal and Hypoxic Brain

Oleate inhibited phosphofructokinase activity in the brain in a way which was similar to known surface‐active protein denaturants. Experimental hypoxia produced an increase in the level of fatty acids in the microsomal, but not in the cytosol fraction of the brain. It is suggested that under hypoxic conditions the influence of fatty acids on the activity of phosphofructokinase (if any) is of non‐specific character.