In this article there are two methods presented for determination of 2 groups of flavonoids: flavone/flavonols (FF) - AlCl 3, and flavanones/dihydroflavonols (FD) - 2,4-dinitr ophenylhidrazine. Together they sum up the total flavonoids (FvT) in propolis. Romanian propolis taken into analysis had an amount of 4% flavone/flavonols, 6% flavanones/dihydroflavonols a nd about 10% total flavonoids typical for propolis derived from Populus sp. Statistical correlation between data (FF, FD, FvT) obtained by sonication and by maceration present no significance difference between results.
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Mărghitaş et al. (2007) studied this question.
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