Ultrathin Multilayered Films that Promote the Release of Two DNA Constructs with Separate and Distinct Release Profiles

‘Charge-shifting’ cationic polymers are used to fabricate ultrathin multilayered films that promote the release of two different DNA constructs with separate and essentially nonoverlapping release profiles. This approach could contribute to the development of thin films and functional coatings capable of regulating the localized release of different DNA constructs (or other agents) to cells or tissues in a broad range of fundamental and applied contexts.