Key result
Laser capture microdissection of surgical islets identifies 11 T2D-linked eQTL genes undetected in organ donors.
Why the study?
Genetic loci identified by GWAS for type 2 diabetes often localize in non-coding regions, suggesting gene regulation effects that require further elucidation through combined genetic and transcriptomic analysis of human islets.
Population
103 metabolically phenotyped pancreatectomized patients and 100 brain-dead organ donors
Comparison
Laser capture microdissection islets vs collagenase digestion islets for eQTL analysis
Design
Observational study combining genotyping and expression analyses
Authors
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May highlight limitations of donor-based islet studies in type 2 diabetes; leaves open functional roles of these eQTL genes.
Observational (n=203)
eQTL analysis of human pancreatic islets using laser capture microdissection identifies novel genes linked to islet biology and type 2 diabetes.
Khamis et al. (2019) conducted an observational in Type 2 diabetes (n=203). Laser capture microdissection of pancreatic islets from surgical patients vs. Collagenase digestion of pancreas from brain-dead organ donors was evaluated on Identification of cis-eQTLs and differentially expressed genes in T2D. Laser capture microdissection of pancreatic islets from surgical patients identified 11 eQTL genes differentially expressed in type 2 diabetes that were not detected in organ donor samples.
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