⌘+K

Pulse Explore Communities Researchers Journals

Home Communities Explore

October 9, 2004Acta Crystallographica Section F Structural Biology and Crystallization CommunicationsOpen Access

Crystallization and preliminary X-ray characterization of D -3-hydroxybutyrate dehydrogenase fromPseudomonas fragi

Y. Nakajima Kiyoshi Ito Emi Ichihara Kyohei Ogawa Takashi Egawa Yue Xu Tadashi Yoshimoto

View Full Paper

Ask AI

Bookmark

Share

Abstract

A recombinant form of D-3-hydroxybutyrate dehydrogenase (EC 1.1.1.30) from Pseudomonas fragi has been crystallized by the hanging-drop method using PEG 3000 as a precipitating agent. The crystals belong to the orthorhombic group P2(1)2(1)2, with unit-cell parameters a = 64.3, b = 99.0, c = 110.2 A. The crystals are most likely to contain two tetrameric subunits in the asymmetric unit, with a VM value of 3.29 A3 Da(-1). Diffraction data were collected to a 2.0 A resolution using synchrotron radiation at the BL6A station of the Photon Factory.

Discussion

No takes yet. Share an insight, caveat, or question.

Cite This Study

Nakajima et al. (2004) studied this question.

synapsesocial.com/papers/6ab20a6ffbea9e54a1adc6de https://doi.org/10.1107/s1744309104024741

View Full Paper

Ask AI

Bookmark

Share

Keep reading

Synapse has enriched 5 related papers. Consider them for comparative context:

1Crystal structure of the flavin-dependent thymidylate synthase Thy1 from Thermus thermophilus with an extra C-terminal domain.Acta Crystallographica Section F Structural Biology and Crystallization Communications · Sat,
2Noncovalent structure of SENP1 in complex with SUMO2.Acta Crystallographica Section F Structural Biology and Crystallization Communications · Wed,
3A revisited version of the apo structure of the ligand-binding domain of the human nuclear receptor retinoic X receptor α.Acta Crystallographica Section F Structural Biology and Crystallization Communications · Fri,
4The X-ray crystal structure of human endothelin 1, a polypeptide hormone regulator of blood pressure.Acta Crystallographica Section F Structural Biology and Crystallization Communications · Tue,
5Two-dimensional crystallization of intact F-ATP synthase isolated from bovine heart mitochondriaActa Crystallographica Section F Structural Biology and Crystallization Communications · Thu,

More from Acta Crystallographica Section F Structural Biology and Crystallization Communications

Crystallization and preliminary X-ray diffraction analysis of D53H mutantEscherichia colicAMP receptor proteinThu,
Crystallization of the CHAP domain of the endolysin fromStaphylococcus aureusbacteriophage KThu,
Crystallization and preliminary X-ray analysis of the ATPase domain of the σ⁵⁴-dependent transcription activator NtrC1 fromAquifex aeolicusbound to the ATP analog ADP–BeFxThu,
Crystallization and sulfur SAD phasing of AggA, the major subunit of aggregative adherence fimbriae type I from theEscherichia colistrain that caused an outbreak of haemolytic-uraemic syndrome in GermanyThu,
Cloning, expression, purification, crystallization and preliminary X-ray studies of argininosuccinate lyase (Rv1659) fromMycobacterium tuberculosisThu,