Triterpene Glycosides from Sea Cucumber Holothuria leucospilota

Aim To find new bioactive triterpene glycosides from the sea cucumber Holothuria leucospilota . Methods Column chromatography on silica gel and ODS RP-C 18 and HPLC were used for the isolation and purification of triterpene glycosides, and their structures were elucidated on the basis of spectral data and chemical evidence. Results Seven triterpene glycosides were isolated from the sea cucumber H. leucospilota , and identified as leucospilotaside A ( 1 ), leucospilotaside B ( 2 ), leucospilotaside C ( 3 ), holothurin B ( 4 ), holothurin B 2 ( 5 ), echinoside B ( 6 ), and holothurin A ( 7 ). Conclusion Compound 2 is a new triterpene glycoside, and compounds 1, 3, 5 and 6 have been isolated from this sea cucumber for the first time.