A high-throughput crystallization-to-structure pipeline for structural genomics has been developed at Advanced Protein Crystallography Research Group (APCR-group) of RIKEN SPring-8 Center, Japan.The structure determination pipeline includes three elemental technologies for automation developed in RIKEN SPring-8 Center: the automated crystallization and observation robot system TERA; X-ray diffraction data collection using the SPring-8 Precise Automatic Cryosample Exchanger SPACE; automatic X-ray data analysis from phasing to model checking/revision using the Package of Expert Researcher's Operation Network PERON.During five years from 2002, 234 of cryoloop-mountable crystals, 175 of diffraction data sets and 149 of refined structures have been produced from 437 of purified proteins in APCR-group, by seven researchers with assistance of the developed pipeline.Used protocols in this pipeline will be introduced.
No takes yet. Share an insight, caveat, or question.
James M. Holton (2008) studied this question.