The photosensitized formation of NAD(P)H by enzyme-catalysed processes has been accomplished. With Ru(bpy)2+3 as sensitizer, methyl viologen, MV2+ as primary electron acceptor, and (NH4)3EDTA or 2-mercaptoethanol, NADPH is formed in the presence of ferredoxin NADP+-reductase as enzyme catalyst. Zinc(II) meso-tetramethylpyridiniumporphyrin, ZnTMPyP4+ is used as sensitizer for the photoinduced production of NADH using the same components and lipoamide dehydrogenase as enzyme catalyst. The photoinduced NAD(P)H regeneration systems have been coupled to secondary enzyme-catalysed processes such as the reduction of butan-2-one to butan-2-ol, pyruvic acid to lactic acid, acetoacetic acid to β-hydroxybutyric acid, as well as to the reductive amination of pyruvic acid to alanine and of α-oxoglutaric acid to glutamic acid. The products exhibit high optical purity and the enzymes and the coenzymes show high turnover numbers and stability.
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Mandler et al. (1986) studied this question.
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