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Papain was covalently coupled to ZrO2−coated porous glass by several different methods. These derivatives were characterized and their operational half‐lives determined using casein substrate. Papain covalently coupled to the porous glass, previously converted to a carboxylic acid derivative, through amide linkage gave a 35 day operational half‐life.
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Weetall et al. (1973) studied this question.
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