In view of the similarity of TSH and LATS effects on the thyroid, we compared the effects of TSH (NIH-TSH-B4 and Thytropar) and purified LATS-IgG on lipolysis and the metabolism of U-14C-glucose by slices of guinea pig epididymal adipose tissue. Neither TSH nor LATS consistently affected glucose oxidation or incorporation of glucose carbon into total glycerides but both substances stimulated lipolysis and the incorporation of glucose carbon into glyceride-glycerol. Lipolysis was enhanced by TSH in concentrations of 0.010 mU/ml or more and this was inhibited by the addition of antiserum to TSH but not by that to human IgG. LATS-IgG in concentrations of 0.166 mg/ml (an amount per flask that had no significant effect in the mouse bioassay) enhanced lipolysis; a peak effect was seen with 0.66 mg/ml. This stimulation of lipolysis was inhibited by antiserum to human IgG, but not by anti-TSH, and also was inhibited by preincubation of the LATSIgG with 10 gEq of thyroid microsomes, whereas liver microsomes had no effect. These data show that LATS, probably an antibody to thyroid antigen, has marked effects on the metabolism of at least one extrathyroid tissue. (Endocrinology88: 26, 1971)
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Hart et al. (1971) studied this question.