An immunochemical approach for class detection of organophosphorus pesticides was investigated. Synthesis of O, O -diethyl phosphorothionate haptens and their use in the generation of polyclonal antisera are described. An indirect inhibition format ELISA coupled with an automated screening procedure was used to characterize the dose−response of the resulting antisera for 17 representative O, O -diethyl and O, O -dimethyl phosphorothionates/thionothiolates and phosphates.
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Johnson et al. (1998) studied this question.
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