Capture with ketone: 5-Hydroxylmethylcytosine (5-hmC) in DNA can be selectively labeled with a keto-glucose and subsequently linked to biotin via a ketoxime linker for enrichment and detection/sequencing purposes (see scheme). Keto-glucose can be more efficiently transferred than azide-glucose by β-glucosyltransferase, and can provide single-base resolution detection/ sequencing of 5-hmC.
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Song et al. (2011) studied this question.
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