Diagnostic significance of circulating long noncoding RNA PCAT6 in patients with non-small cell lung cancer

Figure 1. Tissue expression of PCAT6 significantly increased in NSCLC patients from GEO datasets. Notes: Tissue expression of PCAT6 in cancer and normal tissues of NSCLC patients from five GEO datasets, which were used on the same platform, GPL570, and conducted on the GeneChip Human Genome U133 Plus 2.0 Array. The probe ID of PCAT6 is 231233_at. PCAT6 expression data was downloaded from GEO (https://www.ncbi.nlm.nih.gov/geo/). *p<0.0001. Tissue expression of PCAT6 was significantly increased in LUAD patients from GSE19804 (A); GSE27262 (B), GSE30219 (D), GSE19188 (E). PCAT6 expression also increased in LUSC patients from GSE30219 (D) and GSE19188 (E). GSE18842 (C) was not described the detailed subtype of NSCLC patients, but PCAT6 expression was increased in total NSCLC patients. Abbreviations: GEO, Gene Expression Omnibus; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; PCAT6, prostate cancer-associated transcript 6.

Figure 2. ROC curves of tissue PCAT6 expression for differentiating NSCLC tissue from normal tissue. Notes: The AUC was calculated. The diagnostic value of PCAT6 for (A–D) LUAD patients and (E–F) LUSC patients in individual GEO datasets. Abbreviations: AUC, area under the ROC curve; GEO, Gene Expression Omnibus; NSCLC, non-small cell lung cancer; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; ROC, receiver operating characteristics; PCAT6, prostate cancer-associated transcript 6.

Figure 3. The tissue expression and diagnostic value of PCAT6 for NSCLC patients from Union Hospital. Notes: Seventy-three paired LUAD tissues and normal counterparts (A), and 51 paired LUSC tissues and normal counterparts (C) were collected from the department of thoracic surgery of Union Hospital (Wuhan, People’s Republic of China). The diagnostic values of tissue PCAT6 expression for LUAD (B) and LUSC (D) were evaluated by qRT-PCR. *p<0.0001. Abbreviations: AUC, area under the receiver operating characteristic curve; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; PCAT6, prostate cancer-associated transcript 6; qRT-PCR, quantitative reverse-transcriptase polymerase chain reaction.

Figure 4. The diagnostic potential of circulating PCAT6 for NSCLC. Notes: (A) The expression of PCAT6 in serum samples from 39 HD, LUAD, and LUSC patients was evaluated by qRT-PCR. *p<0.0001. The diagnostic potential of circulating PCAT6 for LUAD (B) and LUSC (C) was evaluated. Abbreviations: AUC, area under the receiver-operating characteristic curve; HD, healthy donors; LUAD, lung adenocarcinoma; LUSC, lung squamous cell carcinoma; NSCLC, non-small cell lung cancer; PCAT6, prostate cancer-associated transcript 6; qRT-PCR, quantitative reverse-transcriptase polymerase chain reaction.