Extraction, Partial Purification and Characterization of Bromelain from Pineapple (Ananas Comosus) Crown, Core and Peel Waste

HIGHLIGHTS Bromelain was extracted from crown, core, and peel waste of pineapple. Ammonium sulphate (NH4)2SO4 precipitation method was used as a simple and cost-effective approach for partial purification of bromelain. A purification of 4.34, 2.75 and 2.59-fold was achieved for crown, core and peel bromelain samples respectively. Enzyme activity recovery of 170.41 %, 192.37 % and 337.59 % was achieved for crown, core, and peel bromelain, respectively.