Crystal Growth, Vibrational, Optical and Antimicrobial Activity Properties of N-(4-Hydroxyphenyl) Acetamide Nitrate Crystal

Background and Objective:The commonly used medicines of N-(4-hydroxyphenyl) acetamide can help to treat pain and reduce high temperature.It belongs to the classes of analgesic and antipyretic drugs.In this study, the effect of nitric acid on the N-(4-hydroxyphenyl) acetamide crystals obtained from the aqueous ethanol solutions using the slow evaporation method was examined. Materials and Methods:Raw materials of N-(4-hydroxyphenyl) acetamide, nitric acid, ethanol and distilled water were used for this crystallization.The 4-HPAN crystals had been grown with high transparency using the slow evaporation method.The pure crystalline nature was characterized using ultraviolet-visible-near infrared, FT-IR and FT-Raman spectra, powder X-ray diffraction and scanning electron microscopy.Results: Crystallization of 4-HPA in the presence of nitric acid may permit the alteration of crystal shape by solution growth technique.The molecular structure obtained using Chem3D software was connected through the O-H…O hydrogen bond.The FT-IR and FT-Raman spectra confirmed the presence of the functional groups in the synthesized material.Using ultravioletvisible-near infrared spectral analysis, the optical quality of the grown crystal was studied and showed that the crystal has 100% transmittance with the lower cut-off wavelength of 305 nm.The plate-like morphology was confirmed by the scanning electron microscopy.The compound inhibits the growth of certainly tested microorganisms at high concentrations except for Escherichia coli.Conclusion: The synthesized N-(4-hydroxyphenyl) acetamide nitrate crystal has good crystallinity, functional group vibrations, high transparency in the visible region, plate like morphology with increasing oxygen and nitrogen composition and high inhibition against certain bacteria except Escherichia coli.