Quantitative Compositional Analysis of Eggshell Membrane Extracts via 1064 nm Raman Spectroscopy

Eggshell membrane (ESM) extracts are widely utilized as bioactive biomaterials and nutraceuticals due to their rich content of structural proteins and glycosaminoglycans. Ensuring the compositional purity of these materials—specifically the degree of separation between the organic membrane and the inorganic, mineralized eggshell—is critical for processing efficacy and quality control. In this study, 1064 nm near-infrared Raman spectroscopy was employed for high-fidelity, nondestructive compositional analysis. Characteristic Raman signals for carbonate (1085 cm −1 ) and Amide I (1668 cm −1 ) were utilized to differentiate the inorganic shell and organic membrane phases. A robust linear calibration model (R 2 = 0.99) was established between Raman intensity ratios and known mass ratios using laboratory-prepared reference standards. Application of this quantitative methodology to representative consumer-grade ESM powders successfully estimated residual hard-shell mass percentages, demonstrating that 1064 nm Raman spectroscopy provides a rapid, reliable analytical framework for monitoring material purity in ESM processing and manufacturing.