A sporulation mutant of Bacillus subtilis 168 was isolated and characterized. The mutant, designated SB‐23, releases viable forespores at the end of the developmental period. Forespores were isolated on linear Renografin gradients and used as a source of forespore membranes. The protein composition of forespore membranes was found to differ from the protein composition of vegetative cell membranes by discgel electrophoresis. The results are discussed in relationship to morphological and physiological differentiation during bacterial sporulation.
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Robert C. Goldman (1973) studied this question.
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