PISP1, a heteropolysaccharide isolated from fruiting bodies of Phellinus igniarius by hot aqueous extraction and purified by DEAE-Sepharose anion-exchange and gel filtration chromatography, is composed of fucose, galactose, mannose, and 3-O-Me-galactose in a ratio of 1:2:1:2. Methylation, monosaccharide analysis, and NMR studies (1H NMR, 13C NMR, COSY-45 degrees , TOCSY, ROESY, HSQC, and HMBC) revealed that PISP1 had a backbone consisting of 1,6-disubstituted-3-O-Me-alpha-D-galactopyranosyl residue, 1,3,6-trisubstituted-alpha-D-manopyranosyl residue, 1,4-disubstituted-alpha-D-galactopyranosyl residue, and 1,2-disubstituted-alpha-D-galactopyranosyl residue, and had a 1-substituted-alpha-L-fucopyranosyl terminal attached to O-3 of a manopyranosyl residue. Preliminary bioactivity tests conducted in vitro revealed that PISP1 stimulated the proliferation of mouse spleen lymphocytes.
No takes yet. Share an insight, caveat, or question.
Yang et al. (2009) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: