Heterobifunctional fluorescent molecules possessing the same fluorescent properties as their monofunctional parent compounds are investigated Two different functions of these probes do not alter their lasing properties and allow many potential applications in ccllular biochemistry.This paper investigates two families of compounds derived from carbazole and coumarin.The synthesis and spectral propenies of these probes are described.current fluomcentreagents used in the synthesis and application of various fluomgenic substrate^.^.^They possess the basic requirements for use in fluorogenic enzyme substrates which have a relatively high quantum yield and a high shift of the emission spectrum of the free amine upon hydrolysis of the acyl deri~ative.~.Their amine function can react with carboiylic ends of short peptides such as enzyme susbtratcs to form fluomgenic probes leading to a number of biological applications.5-8
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Guillaumet et al. (1992) studied this question.