PCR (polymerase chain reaction) with single, arbitrarily chosen primers was used to detect DNA polymorphisms among 34 sibling gametothalli (single-basidiospore hyphal cultures) and spermatia (single drops of pycniospores) derived from a single-urediniospore culture of the fusiform rust fungus, Cronartium quercuum f. sp. fusiforme. Ten single-basidiospore hyphal cultures were distinguished by comparing nine polymorphic segments produced by four primers. Polymorphisms also were detected between 24 single drops of pycniospores collected from four different galls and drops within the same gall, revealing that phenotypes of galls were all different, and some contained more than one phenotype []
No takes yet. Share an insight, caveat, or question.
Robert L. Doudrick (1993) studied this question.