// Heng Yin 1 , Min Jiang 1 , Xi Peng 1,2 , Hengmin Cui 1,2 , Yi Zhou 2 , Min He 2 , Zhicai Zuo 1,2 , Ping Ouyang 2 , Junde Fan 3 and Jing Fang 1,2 1 Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, Chengdu, Sichuan, PR China 2 College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, PR China 3 College of Biological and Agro-Forestry Engineering, Tongren University, Tongren, Guizhou, PR China Correspondence to: Jing Fang, email: // Keywords : AFB 1 ; G 2 /M phase; cell cycle arrest; mechanism; jejunum; Pathology Section Received : March 11, 2016 Accepted : May 12, 2016 Published : May 25, 2016 Abstract Aflatoxin B 1 (AFB 1 ) has potent hepatotoxic, carcinogenic, genotoxic, immunotoxic and other adverse effects in human and animals. The aim of this study was to investigate the molecular mechanism of G 2 /M cell cycle arrest induced by AFB 1 in the jejunum of broilers. Broilers, as experimental animals, were fed 0.6 mg/kg AFB 1 diet for 3 weeks. Our results showed that AFB 1 reduced the jejunal villus height, villus height/crypt ratio and caused G 2 /M cell cycle arrest. The G 2 /M cell cycle was accompanied by the increase of ataxia telangiectasia mutated (ATM), p53, Chk2, p21 protein and mRNA expression, and the decrease of Mdm2, cdc25C, cdc2, cyclin B and proliferating cell nuclear antigen protein and mRNA expression. In conclusion, AFB 1 blocked G 2 /M cell cycle by ATM pathway in the jejunum of broilers.
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