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March 1, 2003Applied and Environmental MicrobiologyOpen Access

Comparison of Methods for DNA Isolation from Food Samples for Detection of Shiga Toxin-Producing Escherichia coli by Real-Time PCR

Loreé C. Heller Carisa R. Davis K. Kealy Peak David L. Wingfield Andrew C. Cannons Philip T. Amuso Jacqueline Cattani

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Abstract

In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly.

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Heller et al. (2003) studied this question.

synapsesocial.com/papers/6ab34ea7eb183d0301649c0d https://doi.org/10.1128/aem.69.3.1844-1846.2003

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