Analysis of Internal Transcribed Spacer of Ribosomal Dna Reveals Cryptic Speciation in Planoglabratella Opercularis

Research Article| October 01, 2003 ANALYSIS OF INTERNAL TRANSCRIBED SPACER OF RIBOSOMAL DNA REVEALS CRYPTIC SPECIATION IN PLANOGLABRATELLA OPERCULARIS Mashashi Tsuchiya; Mashashi Tsuchiya 1Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. E-mail: tsuchiyam@jamstec.go.jp Search for other works by this author on: GSW Google Scholar Hiroshi Kitazato; Hiroshi Kitazato 2Institute for Frontier Research on Earth Evolution, Japan Marine Science and Technology Center, 2-15 Natsushima-cho, Yokosuka 237-0061, Japan. Search for other works by this author on: GSW Google Scholar Jan Pawlowski Jan Pawlowski 3Department of Zoology and Animal Biology, University of Geneva, 1224 Chêne-Bougeries, Switzerland. Search for other works by this author on: GSW Google Scholar Journal of Foraminiferal Research (2003) 33 (4): 285–293. https://doi.org/10.2113/0330285 Article history received: 22 Apr 2002 accepted: 06 Feb 2003 first online: 03 Mar 2017 Cite View This Citation Add to Citation Manager Share Icon Share Facebook Twitter LinkedIn MailTo Tools Icon Tools Get Permissions Search Site Citation Mashashi Tsuchiya, Hiroshi Kitazato, Jan Pawlowski; ANALYSIS OF INTERNAL TRANSCRIBED SPACER OF RIBOSOMAL DNA REVEALS CRYPTIC SPECIATION IN PLANOGLABRATELLA OPERCULARIS. Journal of Foraminiferal Research 2003;; 33 (4): 285–293. doi: https://doi.org/10.2113/0330285 Download citation file: Ris (Zotero) Refmanager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentBy SocietyJournal of Foraminiferal Research Search Advanced Search Abstract The sequences of internal transcribed spacers of ribosomal DNA (ITS rDNA) are widely used to study the species relationships in different groups of organisms, yet they have not been applied to the study of benthic foraminifera until now. Here, we use the ITS rDNA sequences to examine the phylogenetic relationships between two morphospecies, Planoglabratella opercularis and Planoglabratella nakamurai, collected from around the Japanese Islands. Analysis of 27 ITS rDNA sequences shows that P. opercularis is composed of two genetically distinct species, A and B, the latter one being more closely related to P. nakamurai than to P. opercularis type A. Detailed morphological study confirms our molecular data, which reveals some typical P. nakamurai features in P. opercularis type B. Geographic distribution of both genotypes was examined by using PCR-based RFLP method. Analysis of 58 specimens and sequenced data collected in 10 locations shows that P. opercularis type A is widely distributed throughout the Japanese Islands, while P. opercularis type B is present only in a limited area in the central part of Honshu Island, where it coexists with P. nakamurai and P. opercularis type A. This genetic, morphologic, and geographic evidence suggests that P. opercularis type B is a separate species. Planoglabratella opercularis type B and P. nakamurai are the sister groups of P. opercularis type A. Our study shows that ITS rDNA can be a useful tool for genetic distinction of cryptic species in benthic foraminifera and for studying their intraspecific variation. You do not have access to this content, please speak to your institutional administrator if you feel you should have access.