The goals of this study were to verify the influence of gamma radiation (60Co) and culture medium in the regeneration rate of potato explants, cultivar Macaca, and to molecularly characterize the regenerated plants using RAPD markers. Therefore, internodes and leaves were submitted to different gamma radiation doses and cultivated in basic MS medium with two types of cytokinins, benzylaminopurine (BAP) or zeatin (ZEA). It was observed that internodes, when cultivated in medium with BAP, presented higher regeneration rate when compared to leaves, although decreasing with higher doses. It was also verified that in treatments with ZEA, the internodes presented higher regeneration rate than those treated with BAP, decreasing according to radiation doses. For the molecular analysis, ten RAPD primers were used. These primers produced 70 fragments, being 24% polymorphic. The treatment with 500 cGy promoted the highest variation in genetic distances.
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Wendt et al. (2001) studied this question.
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