Single cell transcriptomics profiling of the stromal cells in the pathologic association of ribosomal proteins in the ischemic myocardium and epicardial fat

Figures

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  Fig. 1. a Representative images for the immunofluorescence analysis for the expression of RPS18 in the LV tissues (1a-I) (N = 3) and the quantification of protein expression of RPS18 in LV tissues calculated from MFI (1a-II). (1a-III). Gene expression analysis of RPS18 in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPS18 in the LVSC (1a-IV) (N = 3) following the treatment with ISC and ISC/R and (1a.V) the quantification of protein expression calculated from MFI. (1a.VI) Representative image of Western blot showing the expression status of RPS18 in the ISC and ISC/R groups compared to the control in LVSC (N = 3) and (1a.VI) quantification represented as fold change relative to GAPDH normalized to control. b Representative images for the immunofluorescence analysis for the expression of RPS18 in EAT (1b-I) (N = 4) and the quantification of protein expression of RPS18 in EAT tissues calculated from MFI (1b-II). (1b-III). Gene expression analysis of RPS18 in EATDS (N = 3) following ISC and ISC/R challenge…

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  Fig. 2. a Representative images for the immunofluorescence analysis for the expression of RPSA in the LV tissues (2a-I) (N = 4) and the quantification of protein expression of RPSA in LV tissues calculated from MFI (2a-II). (2a-III). Gene expression analysis of RPSA in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPSA in the LVSC (2a-IV) (N = 3) following the treatment with ISC and ISC/R and (2a.V) the quantification of protein expression calculated from MFI. (2a.VI) Representative image of Western blot showing the expression status of RPSA in the ISC and ISC/R groups compared to the control in LVSC (N = 3) and (2a.VI) quantification represented as fold change relative to GAPDH normalized to control. b Representative images for the immunofluorescence analysis for the expression of RPSA in the EAT (2b-I) (N = 4) and the quantification of protein expression of RPSA in EAT tissues calculated from MFI (2b-II). (2b-III). Gene expression analysis of RPSA in EATDS (N = 3) following ISC and ISC/R challenge…

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  Fig. 3. a Representative images for the immunofluorescence analysis for the expression of RPL30 in the LV tissues (3a-I) (N = 3) and the quantification of protein expression of RPL30 in LV tissues calculated from MFI (3a-II). (3a-III). Gene expression analysis of RPL30 in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPL30 in the LVSC (3a-IV) (N = 3) following the treatment with ISC and ISC/R and (3a.V) the quantification of protein expression calculated from MFI. (3a.VI) Representative image of Western blot showing the expression status of RPL30 in the ISC and ISC/R groups compared to the control in LVSC (N = 3) and (3a.VI) quantification represented as fold change relative to GAPDH normalized to control. b Representative images for the immunofluorescence analysis for the expression of RPL30 in the EAT (3b-I) (N = 4) and the quantification of protein expression of RPL30 in EAT tissues calculated from MFI (3b-II). (3b-III). Gene expression analysis of RPL30 in EATDS (N = 3) following ISC and ISC/R…

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  Fig. 4. a Representative images for the immunofluorescence analysis for the expression of RPL10A in the LV tissues (4aA-I) (N = 3) and the quantification of protein expression of RPL10A in LV tissues calculated from MFI (4a-II). (4a-III). Gene expression analysis of RPL10A in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPL10A in the LVSC (4a-IV) (N = 3) following the treatment with ISC and ISC/R and (4a.V) the quantification of protein expression calculated from MFI. (4a.VI) Representative image of Western blot showing the expression status of RPL10A in the ISC and ISC/R groups compared to the control in LVSC (N = 3) and (4a.VI) quantification represented as fold-change relative to GAPDH normalized to control. b Representative images for the immunofluorescence analysis for the expression of RPL10A in the EAT (4b-I) (N = 4) and the quantification of protein expression of RPL10A in EAT tissues calculated from MFI (4b-II). (4b-III). Gene expression analysis of RPL10A in EATDS (N = 3) following ISC and…

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  Fig. 5. a Representative images for the immunofluorescence analysis for the expression of RPL14 in the LV tissues (5a-I) (N = 3) and the quantification of protein expression of RPL14 in LV tissues calculated from MFI (5a-II). (5a-III). Gene expression analysis of RPL14 in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPL14 in the LVSC (5a-IV) (N = 3) following the treatment with ISC and ISC/R and (5a.V) the quantification of protein expression calculated from MFI. (5a.VI) Representative image of Western blot showing the expression status of RPL14 in the ISC and ISC/R groups compared to the control in LVSC (N = 3) and (5a.VI) quantification represented as fold change relative to GAPDH normalized to control. b Representative images for the immunofluorescence analysis for the expression of RPL14 in the EAT (5b-I) (N = 4) and the quantification of protein expression of RPL14 in EAT tissues calculated from MFI (5b-II). (5b-III). Gene expression analysis of RPL14 in EATDS (N = 3) following ISC and ISC/R…

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  Fig. 6. a Representative images for the immunofluorescence analysis for the expression of RPS30 (FAU-40) in the LV tissues (6a-I) (N = 3) and the quantification of protein expression of RPS30 in LV tissues calculated from MFI (6a-II). (6a-III). Gene expression analysis of RPS30 in LVSC (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPS30 in the LVSC (6a-IV) (N = 3) following the treatment with ISC and ISC/R and (6a.V) the quantification of protein expression calculated from MFI. b Representative images for the immunofluorescence analysis for the expression of RPS30 in the EAT (6b-I) (N = 4) and the quantification of protein expression of RPS30 in EAT tissues calculated from MFI (6b-II). (6b-III) Gene expression analysis of RPS30 in EATDS (N = 3) following ISC and ISC/R challenge using qRT-PCR relative to the housekeeping gene GAPDH normalized to the control and presented as Log2 FC. Representative images for the immunofluorescence analysis for the expression of RPS30 in the EATDS (6b-IV) (N = 3) following the…