Preparation of the Honeycomb Patterned Porous Film of Biodegradable Polymer for Tissue Engineering Scaffolds

Fabrication and surface modification of a cell scaffold are very important in tissue engineering. In this study, the effects of pore size and surface wettability on hepatocyte adhesion and morphology was studied, using the porous poly-ε-caprolactone (PCL) film prepared by a simple casting technique. The degree of spreading of hepatocyte was enhanced with the decrease of the pore size (~10 μm). Hepatocyte motility and spreading were restricted on the patterned film having cellular-sized pores (ca. 20 μm). Partial hydrolysis of the film surface enhanced the migration and spheroid formation of the cultured hepatocyte.