Plasma‐Activated Water as a Growth Medium in Aeroponic Systems: Impacts on Seed Germination, Plant Physiology, and Biochemistry

Aeroponics offers a sustainable, soil‐less cultivation method with exceptional resource efficiency. This study evaluated plasma‐activated water (PAW) as a bioactive medium for pea ( Pisum sativum L.) under aeroponic conditions. PAW, produced via an air AC DBD plasma jet in ozone mode, contained elevated reactive oxygen and nitrogen species. Compared to deionized water, PAW significantly improved germination, plant height, and biomass. Chlorophyll a, b, and carotenoids rose by 48.2%, 59.6%, and 51.7%; sugars by 81.8% (leaves) and 177.8% (roots); proteins by 66.4% and 156.7%. Oxidative stress and membrane leakage declined, while antioxidant enzymes (SOD, APX, POD, and CAT) were upregulated markedly. PAW enhances growth, biochemical quality, and resilience, demonstrating strong potential as a sustainable, high‐performance medium for precision aeroponic farming.