An ion‐exchange chromatography method is described for the determination of glucosamine and galactosamine hydrolyzed from fungal chitin. The method was designed to handle relatively large samples and to minimize the volume of hydrolyzate needed to be evaporated. Final extracts were analyzed on an automatic amino acid analyzer programmed to inject samples alternately onto two columns. Analysis time on the analyzer was 30 mm per sample. As little as 30 μg of glucosamine per gram of sample was detected.
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HUBBARD et al. (1979) studied this question.
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