Electrochemistry of Cytochrome c Components at Indium Oxide and Promoter Modified Electrodes

Electr〔)n transfer reactions of three components(purified，deamidated and oligomcric)of cytochrome c obtained in the purification procedure havc been examined at both In203and promoter modified electrodes・At an In203elec［rode，the purificd sample gave a we11-defined vokammogram，while dcamidated and oligomeric components of cytochrome c interfered the electrochemical response of purified cytochrome c。On the other hand，a旧hese components showed rapid vokammetric response at promoしcr m〔)difie(i dectτolies．Thc present resuks accountsfortheroleofthe purification procedure ofcytochromec t1)obtain its rapid electron transfer at an In203elcctrode rather than at pr(｝moter modified electrodcs．Also，some pr〔)perties ofdeamidated and oligomeric components ofcytochrome(⊃were ob重ained． l INTRODUCTlON Inrecentyears，electτochemicalstudiesof proteinshavebeenreceivedactivcattention・ Cytochrome c is the most cxtensively studied pr・tcinss・farusedinthisfieldl-3)．Thisis partly because functional electrodes， )n which rapid direct electron transfer of cytochrome c， have been developed， and such conventional electrochemical techniques as cyclic voltammetry havebec・meapPlicable：Indium()xide4・5)and s・一calledpr・m・term・difled6-8)electr・dcsare typical examples・Also，in some cases， bare goldandsilverelcctτ・desarckn・wnt・bc usefulg，10)