Associations of genetic variation and mRNA expression of PDGF / PDGFRB pathway genes with coronary artery disease in the Chinese population

Figures

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  FIGURE 1. Restricted cubic spline regression of the weighted genetic risk score and coronary artery disease. ORs are indicated by solid lines and 95% CIs by shaded areas, which were estimated by logistic regression analyses. Adjusted model: Adjusted for age, gender, smoking, drinking, hypertension, diabetes, and dyslipidemia. CAD, coronary artery disease; CI, confidence interval; OR, odds ratio; wGRS, weighted genetic risk score.

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  FIGURE 2. Distribution of the PDGF/PDGFRB mRNA expression in cases and controls. Vertical black lines in the middle of plots are medians. Mann–Whitney U test was used for mRNA comparisons between cases and controls. The mRNA expression levels of five genes in cases were all significantly lower than those in controls (all p < 0.001). PDGFA, platelet‐derived growth factor A; PDGFB, platelet‐derived growth factor B; PDGFC, platelet‐derived growth factor C; PDGFD, platelet‐derived growth factor D; PDGFRB, platelet‐derived growth factor receptor β.

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  FIGURE 3. Dose–response relationship of PDGF/PDGFRB mRNA expression with AMI by restricted cubic spline regression. (A) restricted cubic spline regression of PDGFA mRNA expression and AMI; (B) restricted cubic spline regression of PDGFB mRNA expression and AMI; (C) restricted cubic spline regression of PDGFC mRNA expression and AMI; (D) restricted cubic spline regression of PDGFD mRNA expression and AMI; (E) restricted cubic spline regression of PDGFRB mRNA expression and AMI. ORs are indicated by solid lines and 95% CIs by shaded areas. Logistic regression models were adjusted for age, gender, smoking, drinking, hypertension, diabetes and dyslipidemia. AMI, acute myocardial infarction; CI, confidence interval; OR, odds ratio; PDGFA, platelet‐derived growth factor A; PDGFB, platelet‐derived growth factor B; PDGFC, platelet‐derived growth factor C; PDGFD, platelet‐derived growth factor D; PDGFRB, platelet‐derived growth factor receptor β.

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  FIGURE 4. PDGFC mRNA expression levels by SNP rs6845322 genotypes. Line plots with median points and quartile error bars. Kruskal–Wallis test was used for the comparison among genotypes. Jonckheere–Terpstra test was used for the trend analysis. **: Significant at 0.01 level, p < 0.01. ns, Not significant, p > 0.05. PDGFC, platelet‐derived growth factor C; SNP, single nucleotide polymorphism.

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  FIGURE 5. Receiver operating characteristic curves of wGRS, PDGF/PDGFRB mRNAs and traditional risk factors for coronary artery disease. Traditional risk factors included age, gender, smoking, drinking, hypertension, diabetes and dyslipidemia. Five genes were PDGFA, PDGFB, PDGFC, PDGFD and PDGFRB. AUC, area under the curve; TRF, traditional risk factors; wGRS, weighted genetic risk score.