Identification of circular RNAs hsa_circ_0044235 in peripheral blood as novel biomarkers for rheumatoid arthritis

Figures

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  Figure 1. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) determined the relative expression levels of circular RNAs (circRNAs) in peripheral blood from rheumatoid arthritis (RA) patients and healthy controls (HC). (a) The average expression of hsa_circ_0010932 did not show any remarkable differences between patients with RA and the HC. (b) The average expression of hsa_circ_0002473 did not show any remarkable differences between patients with RA and the HC. (c) The average expression levels of hsa_circ_0082688 did not show any remarkable differences between patients with RA and the HC. (d) The average expression levels of hsa_circ_0082689 did not show any remarkable differences between patients with RA and the HC. (e) The average expression levels of hsa_circ_0054189 did not show any remarkable differences between patients with RA and the HC. (f) The average expression levels of hsa_circ_0008675 did not show any remarkable differences between patients with RA and the HC. (g) The average expression of hsa_circ_0044235 in patients with RA was significantly more decreased than those of the HC.

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  Figure 2. The expression level of hsa_circ_0044235 in peripheral blood from 77 rheumatoid arthritis (RA) patients was decreased significantly compared to 50 healthy controls (HC).

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  Figure 3. Receiver operating characteristic (ROC) analysis of hsa_circ_0044235 in peripheral blood from rheumatoid arthritis (RA) patients. AUC = area under the curve.

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  Figure 4. The expression of hsa_circ_0044235 in peripheral blood did not show any remarkable differences between new‐ and late‐onset rheumatoid arthritis (RA) patients.

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  Figure 5. Receiver operating characteristic (ROC) curve analysis of the risk‐score of hsa_circ_0044235 in peripheral blood. A The expression level of hsa_circ_0044235 in peripheral blood in rheumatoid arthritis (RA) patients was decreased significantly compared to those of the systemic lupus erythematosus (SLE) controls. (b) ROC curve analysis hsa_circ_0044235 in peripheral blood for the risk‐score in RA patients versus all controls (HC and SLE). (c) ROC curve analysis of hsa_circ_0044235 in peripheral blood for the risk‐score in RA patients versus SLE patients. AUC = area under the curve;

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  Figure 6. Quantitative reverse transcription–polymerase chain reaction (qRT–PCR) determined the relative expression levels of miRNAs in peripheral blood from rheumatoid arthritis (RA) patients and healthy controls (HC). (a) The expression of hsa‐miR‐892a in peripheral blood was increased significantly in RA patients than HC. (b) The expression of hsa‐miR‐135b‐5p did not show any remarkable differences between patients with RA and HC. (c) The expression of hsa‐miR‐135a‐5p did not show any remarkable differences between patients with RA and HC.