The polyaniline-polyvinyl sulfonate-glucose oxidase (PANI-PVS-GOD) electrode has been investigated in the present work. Indium tin oxide (ITO) coated glass electrode was used for the synthesis of polyaniline-polyvinyl sulfonate (PANI-PVS) film using potentiometric method with 0.2 M aniline, 0.5 M PVS solution, 1.0 pH and 1 mA/cm2 current density. The synthesized PANI-PVS composite films were characterized by electrochemical technique, electrical conductivity, UV-visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy and scanning electron microscope (SEM). The GOD was immobilized on synthesized PANI-PVS film by cross-linking via Glutaraldehyde in phosphate and acetate buffer. The sensitivity of PANI-PVS-GOD electrode in phosphate and acetate buffer has been studied. It was found that the phosphate buffer gives fast response as compared to acetate buffer in potentiometric measurements.
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Gaikwad et al. (2007) studied this question.
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