A procedure for rapid multiplication of Chrysanthemum morifolium Ramat by tissue culture techniques is described. Excised stem-tips are placed on semi-solid Murashige and Skoog medium supplemented with growth regulators to form callus and then rotated in the liquid form of the same medium to increase proliferation. The callus pieces are transferred to the same semi-solid medium but kept stationary to induce shoot elongation. The shoots when 2 cm long are transferred to Hoagland’s solution and in a few weeks plantlets are of sufficient size to be planted in soil in the greenhouse. We estimate that 1 chrysanthemum tip could produce 100,000 plantlets in less than 1 year.
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Ben-Jaacov et al. (1972) studied this question.
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