Illudins C2 and C3, New Illudin C Derivatives from Coprinus atramentarius ASI20013.

In the course of screening for antimicrobial antibiotics against Staphylococcus aureus from basidiomycetes, we isolated two active compounds, illudins C2 (1) and C3 (2), from the culture broth of Coprinus atramentarius, which was well known to produce coprine with aldehyde dehydrogenase inhibitory activity1 ~3).This paper presents the fermentation, isolation, structural determination and antimicrobial activities of 1 and 2. The strain ASI20013 grown on potato sucrose agar mediumwas used to inoculate six 500-ml Erlenmeyer flasks containing 100ml of the seed medium consisting of yeast extract 0.4%, malt extract 1.0% and glucose 0.4% (pH 6.0 before sterilization).The flasks were shaken on a rotary shaker for three days at 27°C.The