Key result
Anti-ANP serum increases wet lung weight by ~12% after acid aspiration in rats.
Why the study?
The physiological role of atrial natriuretic polypeptide (ANP) in pulmonary injury induced by HCl aspiration was unclear.
Does injection of anti-ANP serum affect urinary excretion and pulmonary edema in rats with HCl-induced pulmonary injury?
Does injection of anti-ANP serum affect urinary excretion and pulmonary edema in rats with HCl-induced pulmonary injury?
Absolute Event Rate: 0.83% vs 0.74%
p-value: p=<0.05
Endogenous ANP released after pulmonary acid injury plays a physiological role in promoting diuresis and natriuresis, potentially preventing pulmonary edema.
ANP may attenuate edema after acid aspiration in rats; hypothesis-generating and leaves open any human ARDS relevance.
The effect of pulmonary injury induced by aspiration of HCl on plasma atrial natriuretic polypeptide (ANP) level was examined in rats given a constant infusion of water and electrolytes. In addition, using specific antiserum against ANP, we investigated the physiological role of ANP in rats after HCl aspiration. Rats were housed individually in metabolic cages and were given a constant infusion of sodium solution via catheters chronically inserted into the jugular vein. Plasma ANP levels were elevated at 3 and 24 h after tracheal injection of 0.2 ml of 0.1 N HCl via the cricothyroid membrane. Urine volume and urinary sodium excretion increased during the first 24 h after acid aspiration. However, this increase was reduced by the injection of anti-ANP serum. Furthermore, the injection of anti-ANP serum resulted in a significant (P less than 0.05) increase in wet lung weight from a value of 0.74 +/- 0.06 (HCl aspiration with normal rabbit serum injection) to 0.83 +/- 0.07% of body weight. These results indicate that ANP plays a physiological role in the regulation of urinary water and sodium excretion after pulmonary acid injury and suggest that ANP elevated in plasma after pulmonary injury may prevent pulmonary edema with its diuretic action and/or some direct action on water movement in the lung.
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Wakabayashi et al. (1990) studied Pulmonary injury due to HCl aspiration. Anti-ANP serum vs. Normal rabbit serum was evaluated on Wet lung weight (% of body weight) (p=<0.05). Injection of anti-ANP serum in rats after HCl aspiration significantly increased wet lung weight compared to normal rabbit serum (0.83% vs 0.74% of body weight; P<0.05).
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