CircRNA expression profiles and functional analysis in a mouse model of chronic intermittent hypoxia-induced renal injury: new insight into pathogenesis

Figures

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  Figure 1. Differentially expressed circRNAs in renal from CIH mice. (A) The box plot demonstrated the nearly identical distributions of circRNAs in the renal samples of the CIH and Control groups. The red data points are outliers. (B) The scatter plot was used to identify the variation of circRNAs expression between the two groups. The green lines represented 1.5-fold changes. circRNAs beyond the range of green lines indicated >1.5-fold difference between the two compared groups. (C) The volcano plot exhibited significantly aberrant circRNAs in CIH-induced renal injury. The red squares represented the dysregulated circRNAs with significant difference. (D) Hierarchical clustering analysis revealed a distinct circRNA expression pattern between the two groups.

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  Figure 2. Characteristics of altered circRNAs. (A) Chromosomal distribution of the dysregulated circRNAs between the CIH and Control groups. (B) Classification of the significantly altered circRNAs between the two groups.

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  Figure 3. Validation of dysregulated circRNAs. The relative expression levels of six selected circRNAs were determined by qRT-PCR. The upward direction represents gene upregulation. On the contrary, downward direction represents gene downregulation.

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  Figure 4. Functional analysis of altered circRNAs. GO enrichment analysis for upregulated (A) and downregulated (B) circRNAs. KEGG analysis of upregulated (C) and downregulated (D) circRNAs.

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  Figure 5. The ceRNA Network Construction. Light-blue nodes represent protein_coding RNAs, red nodes represent miRNAs, light-green nodes are noncoding RNAs, and brown nodes represent circRNAs.