An assay of lipoxygenase activity was developed that depends on measurements of the time needed for the bleaching of methylene blue to begin. This time was inversely related to the level of lipoxygenase activity (y = 114.5132 - 0.0132x; r= -0.99); it was not affected by the concentration of methylene blue, linoleic acid, or hydroperoxide. Lipoxygenase activity can be calculated from the time needed for the bleaching of methylene blue to begin.
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Toshiyuki Toyosaki (1992) studied this question.