Key result
Post-phlebitic leg syndrome is linked to altered PMN membrane fluidity and heightened cytosolic calcium activation.
Why the study?
The functional alterations of systemic leucocytes in subjects with post-phlebitic leg syndrome (PPS) are not yet clear and require evaluation.
Are there functional alterations in systemic leucocyte rheology at baseline and after in vitro activation in subjects with post-phlebitic syndrome compared to normal controls?
Observational (n=22)
Are there functional alterations in systemic leucocyte rheology at baseline and after in vitro activation in subjects with post-phlebitic syndrome compared to normal controls?
Subjects with post-phlebitic syndrome exhibit functional alterations in systemic leucocytes, including altered filtration and increased cytosolic calcium upon activation.
Hypothesis-generating for leukocyte dysfunction in post-phlebitic syndrome; prospective studies needed before clinical relevance.
Objective: To evaluate leucocyte rheology, expressed as leucocyte filtration, polymorphonuclear leucocyte (PMN) membrane fluidity and cytosolic Ca 2+ concentration in subjects with post-phlebitic leg syndrome (PPS). Methods: In 22 subjects with PPS we determined leucocyte filtration [unfractionated, mononuclear (MN) and PMN cells], employing the St George Filtrometer, PMN membrane fluidity using the fluorescent probe 1-[4-(trimethylamino)phenyl]-6-phenyl-1,3,5-hexatriene (TMA-DPH) and PMN cytosolic Ca 2+ concentration using the fluorescent probe Fura 2-AM. Subsequently we determined the same PMN parameters after in vitro activation with 4-phorbol 12-myristate 13-acetate (PMA) and N -formyl-methionyl-leucyl-phenylalanine (fMLP). Results: At baseline we observed a difference in the filtration parameters of unfractionated and MN cells and an increase in PMN cytosolic Ca 2+ concentration. After activation, a significant variation in PMN filtration parameters was evident both in normals and in PPS subjects, although in subjects with PPS this variation, especially with PMA, was significantly greater. We found a decrease in PMN membrane fluidity and an increase in PMN cytosolic Ca 2+ concentration only in subjects with PPS. Conclusion: These results suggest that there is a functional alteration of systemic leucocytes in PPS, in which the mechanisms are not yet clear.
No takes yet. Share an insight, caveat, or question.
Caimi et al. (1999) conducted an observational in Post-phlebitic leg syndrome (PPS) (n=22). Post-phlebitic leg syndrome vs. Normal subjects was evaluated on Leucocyte filtration, PMN membrane fluidity, and cytosolic Ca2+ concentration. Post-phlebitic leg syndrome was associated with functional alterations of systemic leucocytes, including decreased PMN membrane fluidity and increased PMN cytosolic Ca2+ concentration after activation.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: