Four methods of applying mode-locked lasers to time-resolved fluorescence measurements in the subnanosecond region are compared. When time resolution below 100 ps is not required, the most precise and sensitive method is single-photon counting, and the application of this method to studies of time-resolved fluorescence of tryptophan in simple peptides is described. The dependence of lifetimes on pH and temperature are interpreted in terms of quenching by intramolecular proton and electron transfers.
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Beddard et al. (1980) studied this question.
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