Key result
In vitro studies of SARS-CoV-2 RNA-dependent RNA polymerase reveal that the methods used to produce the enzyme significantly impact its activity and research outcomes.
Highlights the impact of production methods on the enzyme activity of SARS-CoV-2 RdRp in experimental research.
In vitro interrogations of the central replicative complex of SARS-CoV-2, RNA-dependent RNA polymerase (RdRp), by structural, biochemical, and biophysical methods yielded an unprecedented windfall of information that, in turn, instructs drug development and administration, genomic surveillance, and other aspects of the evolving pandemic response. They also illuminated the vast disparity in the methods used to produce RdRp for experimental work and the hidden impact that this has on enzyme activity and research outcomes.
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Wang et al. (2021) studied SARS-CoV-2. Remdesivir Triphosphate and Other Phosphorylated Nucleotides was evaluated. In vitro studies of SARS-CoV-2 RNA-dependent RNA polymerase reveal that the methods used to produce the enzyme significantly impact its activity and research outcomes.
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